Nucleic Acid Kubvisa Kana Kuchenesa Kit
Nucleic Acid Extraction Or Kucheneswa Kitkana kuchengetwa pa -20 ℃. Muenzaniso unofanirwa kutakurwa uchishandisa 0 ℃ curling.
Introduction
Iyo Nucleic Acid Kubvisa Kana Kuchenesa Kit (Magnetic Beads Method) yakagadzirirwa kucheneswa otomatiki kweRNA neDNA kubva kumvura yemuviri (senge swabs, plasma, serum) uchishandisa otomatiki nucleic acid yekubvisa zviridzwa. Magnetic-particle tekinoroji inopa yemhando yepamusoro DNA/RNA iyo inokodzera kushandiswa zvakananga muzasi maapplication akadai sekusimudzira kana kumwe kuita enzymatic.
Application Range
Ropa rose, plasma, serum uye mamwe masampuli ematishu akanyatso lysed uye akagayiwa. Iyo yakaburitswa nucleic asidhi yakasarudzika yakashambadzirwa ne super paramagnetic nanometer magineti bead. Zvadaro mapuroteni, inorganic salt ions uye organic tsvina zvakabviswa nekugeza mhinduro.Pakupedzisira, iyo nucleic acid yakatorwa nemarara kuti iwane yakachena nucleic acid mhinduro.
Kit Contents
| Katsi. Aihwa. | YXN-VIRAL01-32A-BR | Zvikamu | ||
| -50A | - 100A | |||
| Size | 32Tes | 50Test | 100Muedzo | |
| Lysis Buffer | 96 zvakanakaPre-pack ed Plates 2 zvidimbu | 25ml | 50ml | Surfactant uye Tris |
| Wash Buffer I | ★15ml | ★30ml | Mushonga mukuru wemunyu | |
| Wash Buffer Il | ★6ml*2 | ★12ml*2 | Mushonga wakaderera-munyu | |
| Elution Buffer | 10ml | 20ml | Mushonga wakaderera-munyu | |
| MagaBio Reagent | 1.0ml | 2.0ml | Magnetic particles | |
| Handbook(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:ForYXN-VIRAL01-32A-BR-50A,wedzera 15mL Absolute ethanol ku ★15mL Geza Buffer I usati washandisa; wedzera 24mL Absolute ethanol ku ★6mL Geza buffer Il usati washandisa. | ||||
| ForYXN-VIRAL01-32A-BR-100A, wedzera 30mL Absolute ethanol ku ★30mL Wash Buffer I usati washandisa; wedzera 48mL Absolute ethanol ku ★12mL Geza buffer Il usati washandisa.【Zvigadzirwa zvinogadzirwa nemushandisi】Ndokumbira ugadzirire yakakwana ethanol (analytical giredhi) wega. | ||||
Storage Conditions
Pakusvika kwekiti, zvikamu zvekiti zvinogona kuchengetwa panzvimbo yekupisa (15 - 25 ° C). Iyo reagents yakagadzikana kusvika kune rimwe gore kubva pazuva rekugadzira.
Sample Requirements
1. Sample inoshanda: Swabs, plasma, serum uye ropa rose nezvimwe.
2. Sample kuchengetedza uye kutakura: Muenzaniso unofanirwa kuongororwa nekukurumidza
Materials uye Devices Required but Not Provided
1. Magirovhosi asina upfu anoraswa
2. Biohazard mudziyo
3. Penzura kana per
Procedure
Inotevera inoshandisa sampuli yekubvisa swab lotion semuenzaniso kutsanangura muchidimbu matanho ekushanda eiyo yekubvisa reagent pane biological nucleic acid yekubvisa chiridzwa.Bioer NPA-32PkanaSMART 32. Kune mamwe marudzi emuenzaniso, ndapota tarisa kune bhuku rekushandisa. Inogona zvakare kushandiswa nevatengi zvinoenderana nekuyedza kuwana:
1. Reagent Kugadzirira
a. ForYXN-VB03-32A-50A uye YXN-VB03-32A-100A
Wedzera 500uL Lysis Buffer kukoromo 1 ne7 ye2.2mL 96-yakadzika-tsime ndiro, 500uL Wash Buffer I kuchikamu 2 ne8, 500uL Wash Buffer II kuchikamu che3, 4 ne9,10; 70uL Elution Buffer kune mbiru 5 uye 11, 180uL mvura yakachena uye 20uL MagaBio Reagent kune koramu 6 uye 12 (magineti mabedhi anofanira kunyatsosanganiswa asati ashandiswa),
b. ForYXN-VB03-32A
Isa 96 zvakanaka pre-packed reagents pakamuri tembiricha. Zunza 96-tsime ndiro yakatarisa pasi katatu, uye bvarura bhegi repurasitiki. Centrifuge iyo pre-packed reagent kwemasekonzi mashoma (kana kudzungaira neruoko kakati wandei) kudzivirira reagent kunamatira kumadziro emachubhu. Bvarura firimu realuminium foil re96-tsime ndiro uye ratidza kwainoenda ndiro (magnetic beads in column #6 ne#12),
2.Muenzaniso Extraction
1. Wedzera 300uL sampuro kune makumi mapfumbamwe nematanhatu emupureti makoramu #1 uye #7, ndapota dzivirira kusvibiswa-kusvibiswa,
2. Isa 96 yakadzika tsime ndiro kune chiridzwa, isa 8-mitsetse matipi pachiridzwa,
3. Mhanyai chirongwa maererano nemaitiro anotevera,
4. Mushure mekucheneswa otomatiki kwapera, chinjisa elution Buffer mumakoramu 5 uye 11 kune yakachena antinuclear 0.5mL centrifuge chubhu; kana usiri kuishandisa nekukasira, ndapota chengeta pa-20 °C.
Performance Characteristics
1. Chigadzirwa chakabviswa chinowanikwa nepamusoro-sensitivity HBV DNA yekuona reagent kusvika pakunzwa kwe10 IU / mL. Chigadzirwa chakabviswa chinoonekwa nepamusoro-sensitivity HCV RNA yekuona reagent kusvika pakunzwa kwe50 IU/mL.
2. Sarudza 4 samples (serum / plasma samples, nasopharyngeal swab sample, cervical exfoliated cell sample), imwe neimwe sampu inonatswa 10 nguva ne 3 gradients (kusanganisira yekutanga sampuli yakazara ye4 concentrations), vachishandisa vanokwanisa reagents uye test agents kuti vaone mukati. referensi gene zvinoenderana nemirairo yechigadzirwa, uye kukosha kweCt yebatch yega yega inosiyana neisingasviki 1.
| Step | Zvakanaka Nzvimbo | Program Zita | Waiting Time(min:SS) | Mixing Time(mmu:SS) | Magnet Time(min:SS) | Adsorption | Speed | Volume zvinhu(μL) | Temperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Beads | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wmadota1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wash2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wash3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Rasa | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GENERAL KUchengetedzwa.
Kushandisa chigadzirwa ichi nenzira isina kutaurwa muzvinyorwa zvemushandisi zvinogona kukonzera kukuvara kana kukuvadzwa kuchiridzwa kana mudziyo. Ita shuwa kuti chero munhu anoshandisa chigadzirwa ichi awana mirairo mune zvakajairika maitiro ekuchengetedza marabhoritari uye ruzivo rwekuchengetedza rwunopihwa mugwaro iri.
1.1 Usati washandisa chiridzwa kana mudziyo, verenga uye unzwisise ruzivo rwekuchengetedza rwunopihwa mumagwaro emushandisi anopihwa nemugadziri wechiridzwa kana mudziyo.
1.2 Usati wabata makemikari, verenga uye unzwisise ese anoshanda Safety Data Sheets (SDSs) uye shandisa yakakodzera midziyo yekudzivirira yemunhu (magirovhosi, magauni, kudzivirira maziso, nezvimwewo). Kuti uwane maSDS, ona chikamu che "Zvinyorwa uye Tsigiro" mugwaro rino.
2. Chemical kuchengeteka
GENERAL CHEMICAL HANDLING. Kuti uderedze njodzi, ita shuwa kuti vashandi vemurabhoritari vanoverenga uye kudzidzira mirairo yekuchengetedza yekushandiswa kwekemikari, kuchengetedza, uye marara zvakapihwa pazasi, uye bvunza iyo SDS yakakodzera yekudzivirira uye mirairo: Verenga uye unzwisise Safety Data Sheets (SDSs) inopihwa nekemikari. mugadziri usati wachengeta, kubata, kana kushanda nechero makemikari kana zvinhu zvine njodzi..
2.1 Kuderedza kusangana nemakemikari. Pfeka midziyo yekuzvidzivirira yakakodzera pakubata makemikari (somuenzaniso, magirazi ekudzivirira ngozi, magirovhosi, kana zvipfeko zvinodzivirira).
2.2 Kuderedza kufemerwa kwemakemikari. Usasiye midziyo yemakemikari yakashama. Shandisa chete nemhepo yakakwana (somuenzaniso, fume hood).
2.3 Kugara uchitarisa kuti makemikari ari kubuda kana kurasika. Kana pakadonhera kana kupera pakaitika, tevera maitiro ekuchenesa emugadziri sezvaanokurudzirwa muSDS.
2.4 Bata tsvina yemakemikari muhutsi.
2.5 Ita shuwa kushandiswa kwemidziyo yemarara yepuraimari neyechipiri. (Kurasa kwekutanga
mudziyo unobata marara pakarepo. Chigaba chechipiri chine zvinorasika kana kuti zvinobuda kubva mugaba rekutanga. Midziyo yese miviri inofanirwa kuenderana nemarara uye kusangana nemubatanidzwa, nyika, uye zvemuno zvinodiwa zvekuchengetera midziyo.).
2.6 Kana wapedza kubvisa mudziyo wemarara, uvhare nekepisi yawapihwa.
2.7 Shandura (nekuongorora kana zvichidikanwa) tsvina inogadzirwa nemamwe maapplication, reagents, uye substrates inoshandiswa murabhoritari yako.
2.8 Ita shuwa kuti tsvina yachengetwa, kuendeswa, kutakurwa, uye kuraswa maererano nemitemo yese yemunharaunda, yedunhu/dunhu, uye/kana yenyika.
2.9 Radioactive kana biohazardous zvinhu zvingada kubatwa kwakakosha, uye zvipimo zvekurasa zvinogona kushanda.
3.Biological njodzi kuchengeteka
Inogona Biohazard. Zvichienderana nemasampuli anoshandiswa pachiridzwa ichi, nzvimbo yacho inogona kunzi biohazard. Shandisa nzira dzakakodzera dzekubvisa tsvina paunenge uchishanda nebiohazards.
BIOHAZARD. Biological samples dzakadai sematishu, mvura dzemuviri, utachiona, uye ropa revanhu nedzimwe mhuka dzine mukana wekutapurira zvirwere zvinotapukira. Tevedza zvese zvinoshanda zvemuno, dunhu / purovhinzi, uye / kana nyika mitemo. Pfeka midziyo yekudzivirira yakakodzera, inosanganisira asi isingaganhurirwe kune: ziso rekudzivirira, nhovo yechiso, hembe/jasi rebhu, uye magurovhosi. Basa rese rinofanirwa kuitirwa munzvimbo dzakashongedzerwa zvakanaka uchishandisa midziyo yekuchengetedza yakakodzera (semuenzaniso, midziyo yekudzivirira yemuviri). Vanhu vanofanirwa kudzidziswa zvinoenderana nezvinodiwa zvemitemo uye zvekambani / masangano vasati vashanda nezvinhu zvinogona kutapukira.
Verenga uye uteedzere nhungamiro inoshanda uye/kana zvinodikanwa mune zvinotevera:
MuUS: Dhipatimendi reUnited States rehutano neHuman Services gwara rakaburitswa muBiosafety muMicrobiological and Biomedical Laboratories inowanikwa pa: www.cdc.gov/biosafety.
Occupational Safety and Health Standards, Bloodborne Pathogens (29 CFR§1910.1030), inowanikwa pa:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Kambani yako/sangano reBiosafety Chirongwa mapuroteni ekushanda ne/kubata zvinhu zvinogona kutapukira. Rumwe ruzivo nezve biohazard nhungamiro inowanika pa: www.cdc.gov.
MuEU: Tarisai nhungamiro yemuno nemutemo nezve biohazard uye biosafety precaution uye tarisa kune akanakisa maitiro akaburitswa muWorld Health Organisation (WHO) Laboratory Biosafety Manual, chinyorwa chechitatu, chinowanikwa pa: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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